Leukemia Research
Volume 34, Issue 10 , Pages 1325-1329, October 2010

Survivin is upregulated in myeloma cell lines cocultured with mesenchymal stem cells

  • Xiaofang Wang

      Affiliations

    • Department of Hematology, Cancer Hospital of Tianjin, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Ti-Yuan-Bei, Huan-Hu-Xi-Road, Tianjin 300060, PR China
    • Corresponding Author InformationCorresponding author. Tel.: +86 22 23341552; fax: +86 22 23359904.
    • ,
  • Zhiqing Zhang

      Affiliations

    • Department of Neurology, Dalian Central Hospital, Dalian, PR China
    • ,
  • Cheng Yao

      Affiliations

    • Department of Hematology, The First Hospital, Jilin University, Changchun, PR China

Received 17 November 2009; received in revised form 9 May 2010; accepted 11 May 2010. published online 07 March 2011.

Abstract 

In this study we investigated the influence of the mesenchymal stem cells on the survival of U266 and H929 myeloma cell lines. We found that both soluble factors and direct cell contact inhibited daunorubicin-induced apoptosis of U266 and H929 cells. The mRNA and protein levels of survivin were upregulated in U266 and H929 cells cocultured with mesenchymal stem cells, whereas not increased in that separated by TW insert. Antisense oligonucleotides targeting survivin downregulated the expression of survivin and increased the apoptosis of U266 and H929 cells. The results indicated that MSCs had anti-apoptotic effect on U266 and H929 cells that was mediated by survivin.

Abbreviations: MM, multiple myeloma, ECM, extracelluar matrix, MSCs, mesenchymal stem cells, DNR, daunorubicin, TW, transwell, BM, bone marrow, ASODNs, antisense oligonucleotides

Keywords: MSCs, Apoptosis, Survivin, Antisense oligonucleotides, Drug resistance

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PII: S0145-2126(10)00250-X

doi:10.1016/j.leukres.2010.05.006

Leukemia Research
Volume 34, Issue 10 , Pages 1325-1329, October 2010

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