FISH analysis of circulating CD34+ cells as a new tool for genetic monitoring in MDS: Verification of the method and application to 27 MDS patients

Braulke, Friederike; Schanz, Julie; Jung, Klaus; Shirneshan, Katayoon; Schulte, Kristina; Schuetze, Claudia; Steffens, Rainer; Trümper, Lorenz; Haase, Detlef

doi:10.1016/j.leukres.2010.01.010

« Previous Next »Leukemia Research
Volume 34, Issue 10 , Pages 1296-1301, October 2010

FISH analysis of circulating CD34+ cells as a new tool for genetic monitoring in MDS: Verification of the method and application to 27 MDS patients

Friederike Braulke
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Julie Schanz
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Klaus Jung
- Department of Medical Statistics, Georg-August University, Goettingen, Germany
Katayoon Shirneshan
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Kristina Schulte
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Claudia Schuetze
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Rainer Steffens
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Lorenz Trümper
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
Detlef Haase
- Department of Hematology and Oncology, Georg-August University, Robert-Koch-Str. 40, 37075 Goettingen, Germany
- Corresponding author. Tel.: +49 551 39 6313; fax: +49 551 39 12534.

Received 3 November 2009; received in revised form 7 January 2010; accepted 9 January 2010. published online 07 March 2011.

Abstract

In myelodysplastic syndromes (MDS) chromosomal anomalies can be identified in 50–80% of patients. They have a diagnostic and prognostic impact and are increasingly considered for therapeutic decisions. Cytomorphology and cytogenetic analyses of bone marrow (bm) cells define the goldstandard to diagnose MDS patients and to document treatment response. We present a novel method using peripheral blood (pb) for frequent cytogenetic monitoring: after immunomagnetic cell separation circulating CD34+ cells were analysed by fluorescence in situ hybridization (FISH). We compared FISH analyses of enriched and non-enriched pb and bm cells with conventional chromosome banding analyses of bm metaphases: analysing circulating CD34+ cells by FISH is a sensitive, reliable method to measure the abnormal cell clones in pb. This method is practicable, non-invasive, representative for the clonal situation in the bm, and has a predictive value. Its feasibility was proven in a cohort of 27 MDS patients.

Keywords: MDS, CD34+ cells, FISH, Cytogenetics, 5-Azacytidine