Influence of high expression of Smac/DIABLO protein on the clinical outcome in acute myeloid leukemia patients

Pluta, Agnieszka; Wrzesien-Kus, Agata; Cebula-Obrzut, Barbara; Wolska, Anna; Szmigielska-Kaplon, Anna; Czemerska, Magdalena; Pluta, Piotr; Robak, Tadeusz; Smolewski, Piotr; Wierzbowska, Agnieszka

doi:10.1016/j.leukres.2009.11.030

« Previous Next »Leukemia Research
Volume 34, Issue 10 , Pages 1308-1313, October 2010

Influence of high expression of Smac/DIABLO protein on the clinical outcome in acute myeloid leukemia patients

Agnieszka Pluta
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Agata Wrzesien-Kus
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Barbara Cebula-Obrzut
- Department of Experimental Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland
Anna Wolska
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Anna Szmigielska-Kaplon
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Magdalena Czemerska
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Piotr Pluta
- Department of Surgical Oncology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland
Tadeusz Robak
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
Piotr Smolewski
- Department of Experimental Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland
Agnieszka Wierzbowska
- Department of Hematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2 str, 93-510 Lodz, Poland
- Corresponding author. Tel.: +48 426895191; fax: +48 426895192.

Received 5 November 2009; received in revised form 29 November 2009; accepted 29 November 2009. published online 07 March 2011.

Abstract

The role of the Smac/DIABLO protein, a novel apoptosis agonist, in acute myeloid leukemia (AML) is not clearly determined. The expression of Smac/DIABLO protein in AML leukemic cells and its relationship with clinical outcome was evaluated in this study.

The intracellular expression of Smac/DIABLO protein was assessed using multi-color flow cytometry in 71 newly diagnosed AML patients treated with conventional chemotherapy. It was found that the high expression of Smac/DIABLO protein was an independent prognostic factor in terms of higher complete remission rate (p<0.001) and longer overall survival (p=0.003). Moreover the low expression of Smac/DIABLO protein was associated with poor karyotype.