Leukemia Research
Volume 34, Issue 2 , Pages 177-183 , February 2010

Flow cytometric detection of human telomerase reverse transcriptase (hTERT) expression in a subpopulation of bone marrow cells

  • Hiroshi Handa

      Affiliations

    • School of Health Sciences, Gunma University School of Medicine, Maebashi, Japan
    • Corresponding Author InformationCorresponding author at: School of Health Sciences, Gunma University School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan. Tel.: +81 27 220 8166; fax: +81 27 220 8173.
  • ,
  • Takafumi Matsushima

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Natsumi Nishimoto

      Affiliations

    • School of Health Sciences, Gunma University School of Medicine, Maebashi, Japan
  • ,
  • Madoka Inoue

      Affiliations

    • School of Health Sciences, Gunma University School of Medicine, Maebashi, Japan
  • ,
  • Takayuki Saitoh

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Akihiko Yokohama

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Norifumi Tsukamoto

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Takeki Mitsui

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Hirotaka Nakahashi

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Kotaro Toyama

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Masamitsu Karasawa

      Affiliations

    • Blood Transfusion Service, Gunma University Hospital, Maebashi, Japan
  • ,
  • Hatsue Ogawara

      Affiliations

    • School of Health Sciences, Gunma University School of Medicine, Maebashi, Japan
  • ,
  • Yoshihisa Nojima

      Affiliations

    • Department of Medicine and Clinical Science, Gunma University Graduate School of Medicine, Maebashi, Japan
  • ,
  • Hirokazu Murakami

      Affiliations

    • School of Health Sciences, Gunma University School of Medicine, Maebashi, Japan

Received 22 March 2009 ,Revised 8 June 2009 ,Accepted 8 June 2009.

References 

  1. Moyzis RK, Buckingham J, Cram L, Dani M, Deaven L, Jones M, et al. A highly conserved repetitive DNA sequence (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci USA. 1988;85:6622–6626
  2. Blackburn EH. Structure and function of telomeres. Nature. 1991;350:569–573
  3. Harley CB, Kim NW, Prowse KR, Weirich SL, Hirsch KS, West MD, et al. Telomerase, cell immortality, and cancer. Cold Spring Harb Symp Quant Biol. 1994;59:307–310
  4. Landsdorp PM. Telomere length and proliferation potential of hematopoietic stem cells. J Cell Sci. 1995;108:1–6
  5. Hiyama K, Hirai Y, Kyoizumi S, Akiyama M, Hiyama E, Piatyszek MA, et al. Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J Immunol. 1995;155:3711–3715
  6. Counter CM, Gupta J, Harley CB, Leber B, Bacchetti S. Telomerase activity in normal leukocytes and in hematologic malignancies. Blood. 1995;85:2315–2320
  7. Broccoli D, Young JW, de Lange T. Telomerase activity in normal and malignant hematopoietic cells. Proc Natl Acad Sci USA. 1995;92:9082–9086
  8. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PLC, et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994;266:2011–2015
  9. Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, Landsdorp PM. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA. 1994;91:9857–9860
  10. Harley CB, Futcher AB, Greider CW. Telomeres shorten during aging of human fibroblasts. Nature. 1990;345:458–460
  11. Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV, Futcher BA, et al. Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA. 1992;89:10114–10118
  12. Vaziri H, Schachter F, Uchida I, Wei L, Zhu X, Effros R, et al. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet. 1992;52:661–667
  13. Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Allshire RC. Telomere reduction in human carcinoma and with aging. Nature. 1990;346:866–868
  14. Chang E, Harley C. Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci USA. 1995;92:11190–11194
  15. Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB, et al. Telomere shortening associated with chromosome instability in immortal cells which express telomerase activity. EMBO J. 1992;11:1921–1929
  16. de Lange T, Shiue L, Myers RM, Cox DR, Naylor SL, Killery AM, et al. Structure and variability of human chromosome ends. Mol Cell Biol. 1990;10:518–527
  17. Kim NW. Clinical implications of telomerase in cancer. Eur J Cancer. 1997;33:781–786
  18. Say JW, Bacchetti S. A survey of telomerase activity in human cancer. Eur J Cancer. 1997;33:787–791
  19. Hiyama E, Hiyama K. Clinical utility of telomerase in cancer. Oncogene. 2002;21:643–649
  20. Hiyama E, Yokoyama T, Tatsumoto N, Hiyama K, Imamura Y, Murakami Y, et al. Telomerase activity in gastric cancer. Cancer Res. 1995;55:3258–3262
  21. Chiu CP, Dragowska W, Kim NW, Vaziri H, Yui J, Thomas TE, et al. Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow. Stem Cells. 1996;14:239–248
  22. Zhang W, Piatyszek MA, Kobayashi T, Estey E, Andreeff M, Deisseroth AB, et al. Telomerase activity in human acute myelogenous leukemia: inhibition of telomerase activity by differentiation-inducing agents. Clin Cancer Res. 1996;2(5):799–803
  23. Xu D, Gruber A, Bjorkholm M, Peterson C, Pisa P. Suppression of telomerase reverse transcriptase (hTERT) expression in differentiated HL-60 cells: regulatory mechanisms. Br J Cancer. 1999;80:1156–1161
  24. Ohyashiki JH, Sashida G, Tauchi T, Ohyashiki K. Telomeres and telomerase in hematologic neoplasia. Oncogene. 2002;21:680–687
  25. Wright WE, Shay JW, Piatyszek MA. Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity. Nucleic Acids Res. 1995;23:3794–3795
  26. Ohyashiki JH, Ohyashiki K, Toyama K, Shay JW. A nonradioactive, fluorescence-based telomeric repeat amplification protocol to detect and quantitate telomerase activity. Trends Genet. 1996;12(10):395–396
  27. Feng J, Funk WD, Wang SS, Weinrich AAA, Chiu CP, Adams RR, et al. The RNA component of human telomerase. Science. 1995;269:1236–1241
  28. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, et al. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell. 1997;22:785–795
  29. Nakamura TM, Morin GB, Chapman KB, Weinrich SL, Andrews WH, Lingner J, et al. Telomerase catalytic subunit homologs from fission yeast and human. Science. 1997;277(5328):955–959
  30. Takakura M, Kyo S, Kanaya T, Tanaka M, Inoue M. Expression of human telomerase subunits and correlation with telomerase activity in cervical cancer. Cancer Res. 1998;58(7):1558–1561
  31. Hiyama E, Hiyama K, Yokoyama T, Shay JW. Immunohistochemical detection of telomerase (hTERT) protein in human cancer tissues and a subset of cells in normal tissues. Neoplasia. 2001;3(1):17–26
  32. Kyo S, Masutomi K, Maida Y, Kanaya T, Yatabe N, Nakamura M, et al. Significance of immunological detection of human telomerase reverse transcriptase. Re-evaluation of expression and localization of human telomerase reverse transcriptase. Am J Pathol. 2003;163:859–867
  33. Yan P, Benhattar J, Seelentag W, Stehle JC, Bosman FT. Immunohistochemical localization of hTERT protein in human tissues. Histochem Cell Biol. 2004;121:391–397
  34. Wright WE, Piatyszek MA, Rainey WE, Byrd W, Shay JW. Telomerase activity in human germline and embryonic tissues and cells. Dev Genet. 1996;18(2):173–179
  35. Engelhardt M, Kumar R, Albanell J, Pettengell R, Han W, Moore MAS. Telomerase regulation, cell cycle, and telomere stability in primitive hematopoietic cells. Blood. 1997;90:182–193
  36. Engelhardt M, Wasch R, Yalin Guo. Telomeres and telomerase in normal and leukemic hematopoietic cells. Leukemia Res. 2004;28:1001–1004
  37. Ali ASM, Chopra R, Robertson J, Testa NG. Detection of hTERT protein by flow cytometry. Leukemia. 2000;14:2176–2181
  38. Ogawa M. Differentiation and proliferation of hematopoietic stem cells. Blood. 1993;81:2844–2853
  39. Xu D, Gruber A, Peterson C, Pisa P. Telomerase activity and the expression of telomerase components in acute myelogenous leukemia. Br J Haematol. 1998;102:1367–1375
  40. Shiratsuchi M, Muta K, Abe Y, Motomura S, Taguchi F, Takatsuki H, et al. Clinical significance of telomerase activity in multiple myeloma. Cancer. 2002;4:2232–2238

PII: S0145-2126(09)00285-9

doi: 10.1016/j.leukres.2009.06.010

Leukemia Research
Volume 34, Issue 2 , Pages 177-183 , February 2010