OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus

Våtsveen, Thea Kristin; Tian, Erming; Kresse, Stine H.; Meza-Zepeda, Leonardo A.; Gabrea, Ana; Glebov, Oleg; Dai, Hong Yan; Sundan, Anders; Kuehl, W. Michael; Børset, Magne

doi:10.1016/j.leukres.2009.03.001

« Previous Next »Leukemia Research
Volume 33, Issue 12 , Pages 1670-1677, December 2009

OH-2, a hyperdiploid myeloma cell line without an IGH translocation, has a complex translocation juxtaposing MYC near MAFB and the IGK locus

Thea Kristin Våtsveen
- Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
- Department of Pathology and Medical Genetics, St. Olavs University Hospital, Trondheim, Norway
- Corresponding author at: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Olav Kyrres gt. 9, N-7489 Trondheim, Norway. Tel.: +47 73 59 86 60; fax: +47 73 59 88 01.
Erming Tian
- Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
Stine H. Kresse
- Department of Tumor Biology, Radiumhospitalet, Rikshospitalet, Oslo, Norway
Leonardo A. Meza-Zepeda
- Department of Tumor Biology, Radiumhospitalet, Rikshospitalet, Oslo, Norway
- Norwegian Microarray Consortium¹, Department of Molecular Biosciences, University of Oslo, Oslo, Norway
Ana Gabrea
- Genetics Branch, National Cancer Institute, Bethesda, MD, USA
Oleg Glebov
- Genetics Branch, National Cancer Institute, Bethesda, MD, USA
Hong Yan Dai
- Department of Pathology and Medical Genetics, St. Olavs University Hospital, Trondheim, Norway
Anders Sundan
- Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
W. Michael Kuehl
- Genetics Branch, National Cancer Institute, Bethesda, MD, USA
Magne Børset
- Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
- Department of Immunology and Transfusion Medicine, St. Olavs University Hospital, Trondheim, Norway

Received 21 January 2009; received in revised form 21 January 2009; accepted 2 March 2009. published online 07 March 2011.

Abstract

Multiple myeloma can be classified into hyperdiploid (HRD) (with 48–74 chromosomes) and non-hyperdiploid tumors (usually with immunoglobulin heavy chain translocations). The OH-2 human myeloma cell line (HMCL) retains the same HRD genotype as the primary tumor, with extra copies of chromosomes 3, 7, 15, 19, and 21. Both OH-2 and primary cells have a complex secondary translocation in which the IGK 3′ enhancer is inserted between MYC and MAFB, resulting in dysregulation of both oncogenes. OH-2 provides a unique example of an HMCL and the corresponding primary tumor that are shown to share the same HRD genotype.

Keywords: Hyperdiploid, Array CGH, FISH, MYC, MAFB, IGK