Expression of the myeloperoxidase gene in AC133 positive leukemia cells relates to the prognosis of acute myeloid leukemia

Taguchi, Jun; Miyazaki, Yasushi; Tsutsumi, Chizuko; Sawayama, Yasushi; Ando, Koji; Tsushima, Hideki; Fukushima, Takuya; Hata, Tomoko; Yoshida, Shinichiro; Kuriyama, Kazutaka; Honda, Sumihisa; Jinnai, Itsuro; Mano, Hiroyuki; Tomonaga, Masao

doi:10.1016/j.leukres.2005.12.012

« Previous Next »Leukemia Research
Volume 30, Issue 9 , Pages 1105-1112, September 2006

Expression of the myeloperoxidase gene in AC133 positive leukemia cells relates to the prognosis of acute myeloid leukemia

Jun Taguchi
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Yasushi Miyazaki
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
- Corresponding author. Tel.: +81 95 849 7111; fax: +81 95 849 7113.
Chizuko Tsutsumi
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Yasushi Sawayama
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Koji Ando
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Hideki Tsushima
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Takuya Fukushima
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Tomoko Hata
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Shinichiro Yoshida
- Department of Internal Medicine, Nagasaki National Medical Center, 2-1001-1 Ohmura, Nagasaki 856-8562, Japan
Kazutaka Kuriyama
- School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan
Sumihisa Honda
- Department of Radiation Epidemiology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Itsuro Jinnai
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Hiroyuki Mano
- Divisions of Functional Genomics, Jichi Medical School, 3311-1 Minamikawachi-machi Yakushiji, Kawachi-gun, Tochigi 329-0498, Japan
Masao Tomonaga
- Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan

Received 15 October 2005; received in revised form 30 December 2005; accepted 30 December 2005. published online 07 March 2011.

Abstract

We previously reported that the percentage of myeloperoxidase (MPO) positive blasts had a prognostic impact on survival of patients with acute myeloid leukemia (AML). To extend this observation, we quantitatively measured the level of the MPO gene in AC133 positive leukemia cells that would contain a putative AML stem/progenitor compartment. AML cases were divided into the MPO gene high (MPOg-H) and MPO gene low (MPOg-L) groups. Only patients belonging to the MPOg-H group had a favorable chromosomal translocation, t(8;21), and having no morphological dysplasia that was associated with MPOg-L. The difference in the survival of MPOg-H and MPOg-L was statistically meaningful, demonstrating the possible prognostic impact of the expression of MPO gene in AC133 positive leukemia cells.

Abbreviations: AML, acute myeloid leukemia, FAB, French–American–British, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, MLD, multilineage myelodysplasia, MPO, myeloperoxidase, PBS, phosphate-buffered saline, WBC, White blood cell