Pattern of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangements in Polish pediatric acute lymphoblastic leukemia patients—implications for RQ-PCR-based assessment of minimal residual disease

Dawidowska, Małgorzata; Derwich, Katarzyna; Szczepański, Tomasz; Jółkowska, Justyna; van der Velden, Vincent H.J.; Wachowiak, Jacek; Witt, Michał

doi:10.1016/j.leukres.2006.01.002

« Previous Next »Leukemia Research
Volume 30, Issue 9 , Pages 1119-1125, September 2006

Pattern of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangements in Polish pediatric acute lymphoblastic leukemia patients—implications for RQ-PCR-based assessment of minimal residual disease

Małgorzata Dawidowska
- Institute of Human Genetics, Polish Academy of Sciences, Department of Molecular and Clinical Genetics, Strzeszyńska 32, 60 479 Poznań, Poland
- Department of Pediatric Hematology, Oncology and Hematopoietic Stem Cell Transplantation, University of Medical Sciences, Poznań, Poland
- Corresponding author. Tel.: +48 61 6579218; fax: +48 61 8233235.
Katarzyna Derwich
- Department of Pediatric Hematology, Oncology and Hematopoietic Stem Cell Transplantation, University of Medical Sciences, Poznań, Poland
Tomasz Szczepański
- Department of Pediatric Hematology and Oncology, Silesian Academy of Medicine, Zabrze, Poland
Justyna Jółkowska
- Institute of Human Genetics, Polish Academy of Sciences, Department of Molecular and Clinical Genetics, Strzeszyńska 32, 60 479 Poznań, Poland
Vincent H.J. van der Velden
- Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
Jacek Wachowiak
- Department of Pediatric Hematology, Oncology and Hematopoietic Stem Cell Transplantation, University of Medical Sciences, Poznań, Poland
Michał Witt
- Institute of Human Genetics, Polish Academy of Sciences, Department of Molecular and Clinical Genetics, Strzeszyńska 32, 60 479 Poznań, Poland

Received 17 October 2005; received in revised form 30 December 2005; accepted 2 January 2006. published online 07 March 2011.

Abstract

We studied 23 Polish children with precursor-B-ALL, using PCR-heteroduplex analysis and DNA sequencing, to determine the availability of Ig/TCR gene rearrangements as patient-specific MRD-RQ-PCR targets. We found IGH, IGK-Kde, incomplete TCRD, Vδ2-Jα, TCRG and TCRB rearrangements in 83%, 39%, 61%, 35%, 61% and 13% of patients, respectively. Comparison of Ig/TCR gene rearrangements pattern (frequency and characteristics of rearrangements) in Polish patients with those reported for patients of other European nationalities did not show major differences. These results are the first promising step for further development of MRD study in Polish patients according to current diagnostic standards.

Keywords: ALL, Ig gene rearrangements, TCR gene rearrangements, Minimal residual disease