Histologic and molecular characterizations of megakaryocytic leukemia in mice

Hao, Xingpei; Shin, Min Sun; Zhou, Jeff X.; Lee, Chang Hoon; Qi, Chen Feng; Naghashfar, Zohreh; Hartley, Janet W.; Fredrickson, Torgny N.; Ward, Jerrold M.; Morse, Herbert C.

doi:10.1016/j.leukres.2005.08.021

« Previous Next »Leukemia Research
Volume 30, Issue 4 , Pages 397-406, April 2006

Histologic and molecular characterizations of megakaryocytic leukemia in mice

Xingpei Hao
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
- Corresponding author. Tel.: +1 301 594 2344; fax: +1 301 402 0077.
Min Sun Shin
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Jeff X. Zhou
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Chang Hoon Lee
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Chen Feng Qi
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Zohreh Naghashfar
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Janet W. Hartley
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
Torgny N. Fredrickson
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA
- Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
Jerrold M. Ward
- Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
Herbert C. Morse III
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA

Received 1 June 2005; received in revised form 17 August 2005; accepted 18 August 2005. published online 07 March 2011.

Abstract

Six cases of megakaryocytic leukemia (MKL) were identified and analyzed for morphology and molecular features. MKL were composed of megakaryocyte lineage cells ranging from immature to quite mature cells. VWF, GATA1 and RUNX1 were strongly expressed in megakaryocytes in both normal spleen and MKL as analyzed by immunohistochemistry (IHC). Altered expression of Meis1, Pbx1 and Psen2 and Lef1 in MKL detected with oligonucleotide microarrays was confirmed by qPCR and IHC. This is the first report of spontaneous MKL in mice, defining VWF as a biomarker for diagnosis and suggesting possible involvement of a series of genes in disease pathogenesis.

Keywords: Megakaryocytic leukemia, Mouse, Microarray, Immunohistochemistry, Molecular profiling