Leukemia Research
Volume 30, Issue 2 , Pages 170-177, February 2006

Allelic expression and quantitative RT-PCR study of TAp73 and ΔNp73 in non-Hodgkin's lymphomas

  • Marta Cuadros

      Affiliations

    • Human Genetics Department, Spanish National Cancer Centre (CNIO), Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    • Corresponding Author InformationCorresponding author. Tel.: +34 91 224 69 49; fax: +34 91 224 69 23.
    • ,
  • Gloria Ribas

      Affiliations

    • Human Genetics Department, Spanish National Cancer Centre (CNIO), Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    • ,
  • Victoria Fernández

      Affiliations

    • Human Genetics Department, Spanish National Cancer Centre (CNIO), Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    • ,
  • Carmen Rivas

      Affiliations

    • Pathology Department, Jiménez Díaz Foundation Hospital, Reyes Católicos s/n, 28040 Madrid, Spain
    • ,
  • Javier Benitez

      Affiliations

    • Human Genetics Department, Spanish National Cancer Centre (CNIO), Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    • ,
  • Beatriz Martinez-Delgado

      Affiliations

    • Human Genetics Department, Spanish National Cancer Centre (CNIO), Melchor Fernandez Almagro 3, 28029 Madrid, Spain

Received 4 March 2005; received in revised form 30 June 2005; accepted 30 June 2005. published online 07 March 2011.

Abstract 

p73 shares structural and functional homology to p53. p73 generates different proteins using alternative promoters and splicing which have different biological characteristics. We investigated the pattern (monoallelic or biallelic) of expression of TAp73 and ΔNp73 in normal lymphocytes and lymphomas using two p73 polymorphisms. We found monoallelic expression of TAp73 in normal lymphocytes and tumors, and a selective expression of AT allele in all cases. Moreover, the quantitative expression analysis revealed ΔNp73 over-expression in both B- and T-cell lymphomas comparing with normal lymphoid cells, suggesting a role in tumorigenesis. Finally, we have confirmed that although ΔNp73 over-expression could be an alternative mechanism of p53 inactivation, both alterations may appear together.

Keywords: p73, Isoform, Quantitative, Lymphomas, Imprinting, Expression

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PII: S0145-2126(05)00275-4

doi:10.1016/j.leukres.2005.06.024

Leukemia Research
Volume 30, Issue 2 , Pages 170-177, February 2006

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