Leukemia Research
Volume 29, Issue 8 , Pages 923-931, August 2005

JAK–STAT signaling involved in phorbol 12-myristate 13-acetate- and dimethyl sulfoxide-induced 2′-5′ oligoadenylate synthetase expression in human HL-60 leukemia cells

  • Shenhav Cohen

      Affiliations

    • Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
  • ,
  • Sara Dovrat

      Affiliations

    • Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
  • ,
  • Ronit Sarid

      Affiliations

    • Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
    • Corresponding Author InformationCorresponding author. Tel.: +972 3 5317853; fax: +972 3 5351824.
  • ,
  • Eliezer Huberman

      Affiliations

    • Biochip Technology Center, Argonne National Laboratory, Argonne, IL 60439-4833, USA
  • ,
  • Samuel Salzberg

      Affiliations

    • Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
    • Deceased (1940–2000).

Received 6 December 2004; received in revised form 20 January 2005; accepted 31 January 2005. published online 07 March 2011.

Abstract 

The JAK–STAT signal transduction cascade participates in various cellular processes, including immune response, cell replication, differentiation and oncogenesis. Here, we report that this cascade is induced in two human myeloid HL-60 leukemia cell variants by the granulocyte differentiation inducer dimethyl sulfoxide (DMSO) and macrophage differentiation inducer phorbol 12-myristate 13-acetate (PMA). DMSO and PMA also induced the expression and catalytic activity of 2′-5′ oligoadenylate synthetase (2-5A synthetase), a known interferon (IFN) inducible enzyme. The HL-60 cell variants included HL-205, which is susceptible to DMSO- and PMA-induced differentiation, and HL-525, which is susceptible to DMSO- but not to PMA-induced differentiation. Treatment of HL-205 and HL-525 cells with DMSO and HL-205 cells with PMA-induced JAK1 phosphorylation, JAK1/STAT1 association, formation of STAT1–STAT2 heterodimers, and the binding of the active IFN stimulating growth factor 3 (ISGF3) to the IFN-stimulated response element (ISRE) fragment isolated from the 2-5A synthetase promoter. These events were either reduced or absent in the resistant HL-525 cells treated with PMA. Taken together, our data implicate the above signaling cascade in DMSO- and PMA-induced 2-5A synthetase expression and catalytic activity in the HL-60 cell system.

Keywords: HL-60, Interferon-stimulated gene factor complex, Interferon stimulated response element, JAK/STAT signaling, 2′-5′ Oligoadenylate synthetase, DMSO, PMA, Protein kinase C, STAT1, STAT2, Differentiation

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PII: S0145-2126(05)00062-7

doi:10.1016/j.leukres.2005.01.015

Leukemia Research
Volume 29, Issue 8 , Pages 923-931, August 2005