Retroviral expression screening of oncogenes in natural killer cell leukemia

Choi, Young Lim; Moriuchi, Ryozo; Osawa, Mitsujiro; Iwama, Atsushi; Makishima, Hideki; Wada, Tomoaki; Kisanuki, Hiroyuki; Kaneda, Ruri; Ota, Jun; Koinuma, Koji; Ishikawa, Madoka; Takada, Shuji; Yamashita, Yoshihiro; Oshimi, Kazuo; Mano, Hiroyuki

doi:10.1016/j.leukres.2005.02.002

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Volume 29, Issue 8 , Pages 943-949, August 2005

Retroviral expression screening of oncogenes in natural killer cell leukemia

Young Lim Choi
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
- Division of Hematology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan
Ryozo Moriuchi
- Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Medicine, Nagasaki, Japan
Mitsujiro Osawa
- Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Atsushi Iwama
- Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Hideki Makishima
- Second Department of Internal Medicine, Shinshu University School of Medicine, Nagano, Japan
Tomoaki Wada
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Hiroyuki Kisanuki
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Ruri Kaneda
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Jun Ota
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
- CREST, Japan Science and Technology Agency, Saitama, Japan
Koji Koinuma
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Madoka Ishikawa
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Shuji Takada
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Yoshihiro Yamashita
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
Kazuo Oshimi
- Division of Hematology, Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan
Hiroyuki Mano
- Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan
- CREST, Japan Science and Technology Agency, Saitama, Japan
- Corresponding author. Tel.: +81 285 58 7449; fax: +81 285 44 7322.

Received 17 October 2004; accepted 22 January 2005. published online 07 March 2011.

Abstract

Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens (n=8).