Leukemia Research
Volume 29, Issue 8 , Pages 901-913 , August 2005

Nuclear topography and expression of the BCR/ABL fusion gene and its protein level influenced by cell differentiation and RNA interference

  • Eva Bártová

      Affiliations

    • Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • ,
  • Andrea Harničarová

      Affiliations

    • Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • ,
  • Jiří Pacherník

      Affiliations

    • Center for Cell Therapy and Tissue Repair, Charles University, V úvalu 84, 150 06 Prague 5, Czech Republic
    • Laboratory of Molecular Embryology, Mendel University Brno, Zemĕdĕlská 1, 613 00 Brno, Czech Republic
    • ,
  • Stanislav Kozubek

      Affiliations

    • Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
    • Corresponding Author InformationCorresponding author. Tel.: +420 5 41517139; fax: +420 5 41240498.

Received 12 November 2004

References 

  1. Cremer T, Cremer C. Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat Rev Genet. 2001;2:292–301
  2. Dundr M, Misteli T. Functional architecture in the cell nucleus. Biochem J. 2001;356:297–310
  3. Croft JA, Bridger JM, Boyle S, Perry P, Teague P, Bickmore WA. Differences in the localization and morphology of chromosomes in the human nucleus. J Cell Biol. 1999;145:1119–1131
  4. Sadoni N, Langer S, Fauth C, Bernardi G, Cremer T, Turner BM, et al. Nuclear organization of mammalian genomes. Polar chromosome territories build up functionally distinct higher order compartments. J Cell Biol. 1999;146:1211–1226
  5. Kozubek S, Lukášová E, Jirsová P, Koutná I, Kozubek M, Gaňová A, et al. 3D structure of the human genome: order in randomness. Chromosoma. 2002;111:321–331
  6. Kurz A, Lampel S, Nickolenko JE, Bradl J, Benner A, Zirbel RM, et al. Active and inactive genes localize preferentially in the periphery of chromosome territories. J Cell Biol. 1996;135:1195–1205
  7. Verschure PJ, van Der Kraan I, Manders EM, van Driel R. Spatial relationship between transcription sites and chromosome territories. J Cell Biol. 1999;147:13–24
  8. Rice JC, Allis CD. Histone methylation versus histone acetylation: new insights into epigenetic regulation. Curr Opin Cell Biol. 2001;13:263–273
  9. Misteli T. Protein dynamics: implications for nuclear architecture and gene expression. Science. 2001;291:843–847
  10. Rowley JD. Letter: a new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature. 1973;243:290–293
  11. Rowley JD. Identification of the constant chromosome regions involved in human hematologic malignant disease. Science. 1982;216:749–751
  12. Bartram CR, de Klein A, Hagemeijer A, van Agthoven T, Geurts van Kessel A, Bootsma D, et al. Translocation of c-ab 1 oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukaemia. Nature. 1983;306:277–280
  13. Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 1975;45:321–334
  14. Groffen J, Heisterkamp N, Stephenson JR, van Kessel AG, de Klein A, Grosveld G, et al. c-sis is translocated from chromosome 22 to chromosome 9 in chronic myelocytic leukemia. J Exp Med. 1983;158:9–15
  15. Grosveld G, Verwoerd T, van Agthoven T, de Klein A, Ramachandran KL, Heisterkamp N, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol Cell Biol. 1986;6:607–616
  16. Wu SQ, Voelkerding KV, Sabatini L, Chen XR, Huang J, Meisner LF. Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562. Leukemia. 1995;9:858–862
  17. Naumann S, Reutzel D, Speicher M, Decker HJ. Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization. Leuk Res. 2001;25:313–322
  18. Pettiford SM, Herbst R. The protein tyrosine phosphatase HePTP regulates nuclear translocation of ERK2 and can modulate megakaryocytic differentiation of K562 cells. Leukemia. 2003;17:366–378
  19. Choi HJ, Chung TW, Kang NY, Kim KS, Lee YC, Kim CH. Involvement of CREB in the transcriptional regulation of the human GM3 synthase (hST3Gal V) gene during megakaryocytoid differentiation of human leukemia K562 cells. Biochem Biophys Res Commun. 2004;313:142–147
  20. Lindner I, Kharfan-Dabaja MA, Ayala E, Kolonias D, Carlson LM, Beazer-Barclay Y, et al. Induced dendritic cell differentiation of chronic myeloid leukemia blasts is associated with down-regulation of BCR-ABL. J Immunol. 2003;171:1780–1791
  21. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998;391:806–811
  22. Wilda M, Fuchs U, Wossmann W, Borkhardt A. Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene. 2002;21:5716–5724
  23. Bártová E, Kozubek S, Kozubek M, Jirsová P, Lukášová E, Skalníková M, et al. Nuclear topography of the c-myc gene in human leukemic cells. Gene. 2000;244:1–11
  24. Bártová E, Jirsová P, Fojtová M, Souček K, Kozubek S. Chromosomal territory segmentation in apoptotic cells. Cell Mol Life Sci. 2003;60:979–990
  25. Bártová E, Kozubek S, Jirsová P, Kozubek M, Lukášová E, Skalníková M, et al. Higher-order chromatin structure of human granulocytes. Chromosoma. 2001;10:360–370
  26. Bártová E, Kozubek S, Jirsová P, Kozubek M, Gajová H, Lukášová E, et al. Nuclear structure and gene activity in human differentiated cells. J Struct Biol. 2002;139:76–89
  27. Kozubek S, Lukášová E, Rýznar L, Kozubek M, Lišková A, Govorun RD, et al. Distribution of ABL and BCR genes in cell nuclei of normal and irradiated lymphocytes. Blood. 1997;89:4537–4545
  28. Hughes TP, Morgan GJ, Martiat P, Goldman JM. Detection of residual leukemia after bone marrow transplant for chronic myeloid leukemia: role of polymerase chain reaction in predicting relapse. Blood. 1991;77:874–878
  29. Perego RA, Bianchi C, Brando B, Urbano M, Del Monte U. Increment of nonreceptor tyrosine kinase Arg RNA as evaluated by semiquantitative RT-PCR in granulocyte and macrophage-like differentiation of HL-60 cells. Exp Cell Res. 1998;245:146–154
  30. Kozubek M, Kozubek S, Lukášová E, Marečková A, Bártová E, Skalníková M, et al. High-resolution cytometry of FISH dots in interphase cell nuclei. Cytometry. 1999;36:279–293
  31. Melo JV. The molecular biology of chronic myeloid leukaemia. Leukemia. 1996;10:751–756
  32. Shtivelman E, Lifshitz B, Gale RP, Roe BA, Canaani E. Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene. Cell. 1986;47:277–284
  33. Neves H, Ramos C, da Silva MG, Parreira A, Parreira L. The nuclear topography of ABL, BCR, PML, and RARalpha genes: evidence for gene proximity in specific phases of the cell cycle and stages of hematopoietic differentiation. Blood. 1999;93:1197–1207
  34. Bianchi N, Osti F, Rutigliano C, Corradini FG, Borsetti E, Tomassetti M, et al. The DNA-binding drugs mithramycin and chromomycin are powerful inducers of erythroid differentiation of human K562 cells. Br J Haematol. 1999;104:258–265
  35. Gambari R, del Senno L, Barbieri R, Viola L, Tripodi M, Raschella G, et al. Human leukemia K562 cells: induction of erythroid differentiation by 5-azacytidine. Cell Differ. 1984;14:87–97
  36. Cheng XD, Xie HY, Qian RL. Effect of IL-3 on globin gene expression in a human erythroleukemia cell line. Acta Biochem Biophys Sin. 1999;31:385–389
  37. Itoh N, Shimizu N. DNA replication-dependent intranuclear relocation of double minute chromatin. J Cell Sci. 1998;111:3275–3285
  38. Chaly N, Munro SB. Centromeres reposition to the nuclear periphery during L6E9 myogenesis in vitro. Exp Cell Res. 1996;223:274–278
  39. Yang KD, Shaio MF. Hydroxyl radicals as an early signal involved in phorbol ester-induced monocytic differentiation of HL60 cells. Biochem Biophys Res Commun. 1994;200:1650–1657
  40. McGahon A, Bissonnette R, Schmitt M, Cotter KM, Green DR, Cotter TG. BCR-ABL maintains resistance of chronic myelogenous leukemia cells to apoptotic cell death. Blood. 1994;83:3835
  41. Solovyan V, Bezvenyuk Z, Huotari V, Tapiola T, Suuronen T, Salminen A. Distinct mode of apoptosis induced by genotoxic agent etoposide and serum withdrawal in neuroblastoma cells. Brain Res Mol Brain Res. 1998;62:43–55

PII: S0145-2126(05)00055-X

doi: 10.1016/j.leukres.2005.01.011

Leukemia Research
Volume 29, Issue 8 , Pages 901-913 , August 2005

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