Leukemia Research
Volume 29, Issue 6 , Pages 673-677, June 2005

A morphometric approach for the evaluation of angiogenesis in acute lymphoblastic leukemia of childhood

  • Lida Kalmanti

      Affiliations

    • Department of Medical Oncology, University Hospital of Heraklion, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • ,
  • Konstantina Dampaki

      Affiliations

    • Department of Pathology, University Hospital of Heraklion, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • ,
  • Helen Dimitriou

      Affiliations

    • Departments of Pediatric Hematology-Oncology, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • ,
  • Eftichia Stiakaki

      Affiliations

    • Departments of Pediatric Hematology-Oncology, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • ,
  • Vretos Chaniotis

      Affiliations

    • Department of Pathology, University Hospital of Heraklion, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • ,
  • Efstathios Stathopoulos

      Affiliations

    • Department of Pathology, University Hospital of Heraklion, P.O. Box 1352, 71110 Heraklion Crete, Greece
    • Corresponding Author InformationCorresponding author. Tel.: +30 2810394692; fax: +30 2810394694.

Received 18 May 2004; accepted 25 August 2004. published online 07 March 2011.

Abstract 

Angiogenesis was estimated in childhood acute lymphoblastic leukemia (ALL) by the use of a novel morphometric method. This was compared with the widely used one of microvessel density (MVD). Bone marrow biopsies were evaluated by immunostaining with anti-Factor VIII related antigen (FVIIIRAg). Angiogenesis was calculated as volume corrected microvessel density index (VC-MVDI), taking into account the bone marrow cellularity. According to our results both MVD and VC-MVDI were increased at diagnosis of ALL in comparison with the control group. However the VC-MVDI increment was not statistically significant. Therefore, VC-MVDI could be more representative of the true increase of angiogenesis, correlating better with the outcome of the disease.

Keywords: Angiogenesis, Lymphoblastic leukemia, Morphometry, Hot spots, Childhood

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PII: S0145-2126(05)00036-6

doi:10.1016/j.leukres.2004.08.013

Leukemia Research
Volume 29, Issue 6 , Pages 673-677, June 2005

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