Cytotoxic effects of novel amphiphilic dimers consisting of 5-fluorodeoxyuridine and arabinofuranosylcytosine in cross-resistant H9 human lymphoma cells

Saiko, Philipp; Horvath, Zsuzsanna; Illmer, Christoph; Madlener, Sibylle; Bauer, Wolfgang; Hoechtl, Thomas; Erlach, Natascha; Grusch, Michael; Krupitza, Georg; Mader, Robert M.; Jaeger, Walter; Schott, Herbert; Agarwal, Ram P.; Fritzer-Szekeres, Monika; Szekeres, Thomas

doi:10.1016/j.leukres.2004.12.015

« Previous Next »Leukemia Research
Volume 29, Issue 7 , Pages 785-791, July 2005

Cytotoxic effects of novel amphiphilic dimers consisting of 5-fluorodeoxyuridine and arabinofuranosylcytosine in cross-resistant H9 human lymphoma cells

Philipp Saiko
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
- Center of Excellence in Clinical and Experimental Oncology (CLEXO) of the Medical University of Vienna, Austria
Zsuzsanna Horvath
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
- Center of Excellence in Clinical and Experimental Oncology (CLEXO) of the Medical University of Vienna, Austria
Christoph Illmer
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Sibylle Madlener
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Wolfgang Bauer
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Thomas Hoechtl
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Natascha Erlach
- Institute of Cancer Research, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria
Michael Grusch
- Institute of Cancer Research, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria
Georg Krupitza
- Institute of Clinical Pathology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Robert M. Mader
- Department of Medicine I, Clinical Division of Oncology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Walter Jaeger
- Department of Pharmaceutical Chemistry, Faculty of Natural Sciences and Mathematics, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria
Herbert Schott
- Department of Organic Chemistry, Faculty of Chemistry and Pharmacy, University of Tuebingen, Auf der Morgenstelle 18, 72076 Tuebingen, Germany
Ram P. Agarwal
- Department of Medicine, Division of Hematology/Oncology, University of Miami, 1600 N.W. 10th Avenue, Miami, FL 33136, USA
Monika Fritzer-Szekeres
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
Thomas Szekeres
- Clinical Institute of Medical & Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria
- Corresponding author. Tel.: +43 1 40400x5365; fax: +43 1 320 33 17.

Received 6 September 2004; accepted 17 December 2004. published online 07 March 2011.

Abstract

Various amphiphilic heterodinucleoside phosphates have recently been synthesized in order to overcome drug resistance. These agents contain 5-fluorodeoxyuridine (5-FdUrd) and arabinofuranosylcytosine (Ara-C). We now investigated the action of two of these novel dimers (# 2 and # 10) in sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells. The dimers were compared with 5-FdUrd and Ara-C for growth inhibition, apoptosis induction, and cell-cycle effects. No significant difference in the cytotoxicity of dimer # 2 could be observed between sensitive and 5-FdUrd/Ara-C cross-resistant H9 cells (IC₅₀ values of 220nM and 200nM, respectively), indicating that further studies with this compound are warranted.