Expression of SOCS3 mRNA in bone marrow cells from CML patients associated with cytogenetic response to IFN-α

Takeuchi, Kazuto; Sakai, Ikuya; Narumi, Hirosi; Yasukawa, Masaki; Kojima, Kensuke; Minamoto, Yoko; Fujisaki, Tomoaki; Tanimoto, Kazushi; Hara, Masamichi; Numata, Akihiko; Gondo, Hisashi; Takahashi, Masuhiro; Fujii, Nobuharu; Masuda, Kozo; Fujita, Shigeru

doi:10.1016/j.leukres.2004.06.006

« Previous Next »Leukemia Research
Volume 29, Issue 2 , Pages 173-178, February 2005

Expression of SOCS3 mRNA in bone marrow cells from CML patients associated with cytogenetic response to IFN-α

Kazuto Takeuchi
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
Ikuya Sakai
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
- Corresponding author. Tel.: +81 89 960 5296; fax: +81 89 960 5299.
Hirosi Narumi
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
Masaki Yasukawa
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
Kensuke Kojima
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan
Yoko Minamoto
- Department of Internal Medicine, Matsuyama Red Cross Hospital, Japan
Tomoaki Fujisaki
- Department of Internal Medicine, Matsuyama Red Cross Hospital, Japan
Kazushi Tanimoto
- Division of Hematology, Ehime Prefectural Central Hospital, Japan
Masamichi Hara
- Division of Hematology, Ehime Prefectural Central Hospital, Japan
Akihiko Numata
- Medicine and Biosystemic Science, Kyushu University Graduate School of Medicine, Japan
Hisashi Gondo
- Medicine and Biosystemic Science, Kyushu University Graduate School of Medicine, Japan
Masuhiro Takahashi
- School of Health Science Medical Laboratory Science, Niigata University Faculty of Medicine, Japan
Nobuharu Fujii
- Department of Internal Medicine II, Okayama University Medical School, Japan
Kozo Masuda
- Department of Internal Medicine II, Okayama University Medical School, Japan
Shigeru Fujita
- First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan

Received 22 March 2004; accepted 8 June 2004.

Abstract

Previously, we have demonstrated that constitutive expression of suppressor of cytokine signaling-3 (SOCS3) affects the sensitivity of chronic myelogenous leukemia (CML) cell lines to interferon-α (IFN-α). In the present study, we analyzed the expression of SOCS3 mRNA in bone marrow cells from patients with CML at diagnosis, with the aid of real-time polymerase chain reaction. SOCS3 mRNA expression in bone marrow cells from CML patients who responded well to IFN-α therapy was significantly lower than that in cells from healthy volunteers and patients who were resistant to IFN-α therapy. Methylation of SOCS3 promoter was absent in bone marrow cells from all CML patients examined. These results indicate that the expression of SOCS3 mRNA is inversely associated with the sensitivity to IFN-α both in vitro and in vivo and that differences in SOCS3 mRNA expression are not due to the methylation status of SOCS3 promoters.

Keywords: SOCS3, Chronic myelogenous leukemia, Real-time polymerase chain reaction, Interferon-α, Methylation