Leukemia Research
Volume 29, Issue 2 , Pages 185-196, February 2005

In vitro crosstalk between fibroblasts and native human acute myelogenous leukemia (AML) blasts via local cytokine networks results in increased proliferation and decreased apoptosis of AML cells as well as increased levels of proangiogenic Interleukin 8

  • Anita Ryningen

      Affiliations

    • Division for Hematology, Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • Corresponding Author InformationCorresponding author. Tel.: +47 55 97 50 00; fax: +47 55 97 29 50.
    • ,
  • Line Wergeland

      Affiliations

    • The University of Bergen, N-5021 Bergen, Norway
    • ,
  • Nils Glenjen

      Affiliations

    • Division for Hematology, Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • ,
  • Bjørn Tore Gjertsen

      Affiliations

    • Division for Hematology, Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • ,
  • Øystein Bruserud

      Affiliations

    • Division for Hematology, Department of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway

Received 16 February 2004; accepted 14 June 2004.

Abstract 

Interactions between native human acute myelogenous leukemia (AML) blasts and nonleukemic cells in the bone marrow microenvironment seem important both for disease development chemosensitivity. Native human AML blasts from consecutive patients were cultured with normal human bone marrow stromal cells and two fibroblast lines (HFL1 and Hs27) separated by a semipermeable membrane. This bidirectional crosstalk via the cytokine network between AML blasts and fibroblasts caused (i) increased proliferation, (ii) mediated antiapoptotic signalling and (iii) increased local levels of proangiogenic IL8.

Keywords: Acute myelogenous leukemia, Fibroblasts, Proliferation, Apoptosis, Interleukin 8

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0145-2126(04)00233-4

doi:10.1016/j.leukres.2004.06.008

Leukemia Research
Volume 29, Issue 2 , Pages 185-196, February 2005

Article Tools

* Image Usage & Resolution