Leukemia Research
Volume 28, Issue 12 , Pages 1303-1312, December 2004

Induction of anti-leukemic cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous myeloblasts

  • Jianlin Gong

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-617-414-1715; fax: +1-617-414-1784.
    • ,
  • Shigeo Koido

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Yoko Kato

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Yasuhiro Tanaka

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Dongshu Chen

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Anna Jonas

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Ilene Galinsky

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Daniel DeAngelo

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • David Avigan

      Affiliations

    • Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Donald Kufe

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Richard Stone

      Affiliations

    • Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA

Received 22 December 2003; accepted 30 March 2004.

Abstract 

Presentation of AML antigens by dendritic cells (DC) could potentially induce a T cell-mediated anti-leukemic immune response. In the present study, we generated DC from adherent (AD-DC) and non-adherent (NAD-DC) myeloblasts obtained from bone marrows of AML patients. Both cell populations displayed morphological, phenotypic and functional properties of DC. The functions of NAD-DC were compared to AD-DC that had been fused with autologous AML blasts (FC/AML). The FC/AML induced greater T cell proliferation and CTL activity against autologous AML blasts (9/10 cases) as compared to NAD-DC. FC/AML may thus represent a promising strategy for DC-based immunotherapy of patients with AML.

Keywords:  Generation of dendritic cells, Fusion of autologous DC and AML blasts, Autologous T cell proliferation, Anti-leukemia immunity

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PII: S0145-2126(04)00125-0

doi:10.1016/j.leukres.2004.03.018

Leukemia Research
Volume 28, Issue 12 , Pages 1303-1312, December 2004

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