Leukemia Research
Volume 28, Issue 10 , Pages 1023-1031, October 2004

Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling

  • Satoshi Yamasaki

      Affiliations

    • Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81-92-642-5947; fax: +81-92-642-5951.
    • ,
  • Motoi Maeda

      Affiliations

    • Terry Fox Laboratory, British Columbia Cancer Agency, 601 West 10th Avenue, Vancouver, BC, Canada V5Z 1L3
    • ,
  • Koichi Ohshima

      Affiliations

    • Department of Pathology, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jounan-ku, Fukuoka 814-0180, Japan
    • ,
  • Masahiro Kikuchi

      Affiliations

    • Department of Pathology, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jounan-ku, Fukuoka 814-0180, Japan
    • ,
  • Teruhisa Otsuka

      Affiliations

    • Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
    • ,
  • Mine Harada

      Affiliations

    • Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

Received 22 December 2003; accepted 23 February 2004.

Abstract 

Interleukin (IL)-15 plays an important role in the survival of human natural killer (NK) cells. We investigated IL-2/15 signaling in NK cell neoplasms from five patients and in five cell lines (NK-92, KHYG-1, SNK-6, HANK1 and MOTN-1) compared to mature peripheral NK cells from 10 healthy subjects. Apoptosis of NK cell lines was prevented by addition of IL-15 in vitro. Blocking IL-2/15Rβ on IL-2-stimulated NK-92 cells resulted in reduced expression of Bcl-XL and phosphorylated Stat5, which paralleled early apoptosis without altering Bcl-2 expression. These data add IL-2/15Rβ to the list of factors important for the survival of NK cell neoplasms.

Abbreviations:  NK, natural killer, HSCT, hematopoietic stem cell transplantation, IL, interleukin, IL-2Rα, IL-2 receptor-α, IL-15Rα, IL-15 receptor-α, IL-2/15Rβ, IL-2/15 receptor-β, IL-2/15Rγ, IL-2/15 receptor-γ, AICD, activation-induced cell death, HTLV-1, human T-cell leukemia virus-1

Keywords:  Human natural killer cell neoplasms, Interleukin-2, Interleukin-15, Interleukin-15 receptors, Apoptosis, Bcl-XL

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PII: S0145-2126(04)00057-8

doi:10.1016/j.leukres.2004.02.006

Leukemia Research
Volume 28, Issue 10 , Pages 1023-1031, October 2004

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