Leukemia Research
Volume 28, Issue 8 , Pages 821-829, August 2004

Inhibition of acute myeloid leukemia cell growth by mono-specific and bi-specific anti-CD33 × anti-CD64 antibodies

  • Larisa Balaian
    • ,
  • Edward D Ball

      Affiliations

    • Corresponding Author InformationCorresponding author. Present address: Blood and Marrow Transplantation Division, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0960, USA. Tel.: +1-858-657-7053; fax: +1-858-657-6837.

Department of Medicine and Cancer Center, University of California, San Diego School of Medicine, La Jolla, CA, USA

Received 29 July 2003; accepted 2 December 2003.

Abstract 

Bi-specific anti-CD33 × anti-CD64 antibodies (BsAb) mediated more potent and longer-lasting inhibition of proliferation of human leukemia cell lines and primary acute myeloid leukemia (AML) samples compared to mono-specific anti-CD33 mAb. There were no differences between these two antibodies in cellular internalization over time. The inhibitory effect of BsAb was mimicked by a mouse IgG2a subclass mono-specific anti-CD33 mAb. These findings indicate that enhanced inhibition of proliferation was caused by simultaneous ligation of both CD33 and CD64 molecules. We conclude that inhibition of leukemia cell growth initiated by BsAb during prolonged exposure may have therapeutic value for the treatment of AML.

Keywords:  Myeloid leukemia, Bispecific antibodies, CD33 receptor

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0145-2126(03)00412-0

doi:10.1016/j.leukres.2003.12.001

Leukemia Research
Volume 28, Issue 8 , Pages 821-829, August 2004

Article Tools

* Image Usage & Resolution