The distinct gene expression profiles of chronic lymphocytic leukemia and multiple myeloma suggest different anti-apoptotic mechanisms but predict only some differences in phenotype

Zent, Clive S; Zhan, Fenghuang; Schichman, Steven A; Bumm, Klaus H.W; Lin, Pei; Chen, James B; Shaughnessy, John D

doi:10.1016/S0145-2126(03)00015-8

« Previous Next »Leukemia Research
Volume 27, Issue 9 , Pages 765-774, September 2003

The distinct gene expression profiles of chronic lymphocytic leukemia and multiple myeloma suggest different anti-apoptotic mechanisms but predict only some differences in phenotype

Clive S Zent
- Division of Hematology/Oncology, Central Arkansas Healthcare System and University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Department of Pathology and Laboratory Medicine Service, Central Arkansas Healthcare System and University of Arkansas for Medical Sciences, 113/LR, 43001 W. 7th Street, Little Rock, AR 72205–5484, USA
- Corresponding author. Address from 1st April 2003: Division of Hematology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. Tel.: +1-507-284-2511; fax: +1-507-266-4972.
Fenghuang Zhan
- Donna and Donald Lambert Laboratory of Myeloma Genetics, Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Tel.: +1-501-296-1503x1410; fax: +1-501-686-6442.
Steven A Schichman
- Department of Pathology and Laboratory Medicine Service, Central Arkansas Healthcare System and University of Arkansas for Medical Sciences, 113/LR, 43001 W. 7th Street, Little Rock, AR 72205–5484, USA
- Tel.: +1-501-257-6445; fax: +1-501-257-6441.
Klaus H.W Bumm
- Donna and Donald Lambert Laboratory of Myeloma Genetics, Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Tel.: +1-501-296-1503x1410; fax: +1-501-686-6442.
Pei Lin
- Department of Pathology, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Tel.: +1-501-686-7647; fax: +1-501-686-5874.
James B Chen
- Division of Hematology/Oncology, Central Arkansas Healthcare System and University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Tel.: +1-501-257-1000x55925; fax: +1-501-257-4545.
John D Shaughnessy
- Donna and Donald Lambert Laboratory of Myeloma Genetics, Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205, USA
- Tel.: +1-501-296-1503x1457; fax: +1-501-686-6442.

Received 23 May 2002; accepted 21 December 2002.

Abstract

We compared gene expression in purified tumor cells from untreated patients with chronic lymphocytic (CLL) (n=24) and newly diagnosed multiple myeloma (MM) (n=29) using the Affymetrix HuGeneFL microarray with probes for approximately 6800 genes. Hierarchical clustering analysis showed that CLL and MM have distinct expression profiles (class prediction). Gene and protein expression (measured by flow cytometry) correlated well for CD19, CD20, CD23, and CD138 in CLL and MM, but not for immunoglobulin light chain, CD38 and CD79b in CLL, or CD45 and CD52 in MM. CLL and MM differentially expressed 18% of 130 apoptosis related genes, suggesting differences in mechanisms of cell survival.

Abbreviations: BC, tonsillar B lymphocytes, CD, cluster of differentiation, CLL, chronic lymphocytic leukemia, MM, multiple myeloma, PC, normal bone marrow plasma cells, mRNA, messenger ribonucleic acid, WRS, Wilcoxon rank sum test