Leukemia Research
Volume 27, Issue 9 , Pages 823-829 , September 2003

Influence of protein phosphatase inhibitors on HL60 cells death induction by dehydrocrotonin

  • Ana Claudia Galvão Freire

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Cristiane Fernandes de Assis

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Alexander Otto Frick

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Patrı́cia da Silva Melo

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Marcela Haun

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Hiroshi Aoyama

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
  • ,
  • Nelson Durán

      Affiliations

    • Biological Chemistry Laboratory, Institute of Chemistry, State University of Campinas (UNICAMP), CP 6154, Campinas 13081-970, São Paulo, Brazil
  • ,
  • Mariana Melillo Sauer

      Affiliations

    • Department of Infectoparasitaries Diseases (DIPA), Immunology Laboratory, EPM/UNIFESP, São Paulo, Brazil
  • ,
  • Ésper Georges Kallás

      Affiliations

    • Department of Infectoparasitaries Diseases (DIPA), Immunology Laboratory, EPM/UNIFESP, São Paulo, Brazil
  • ,
  • Carmen Verı́ssima Ferreira

      Affiliations

    • Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), CP 6109, Campinas 13083-970, São Paulo, Brazil
    • Corresponding Author InformationCorresponding author. Fax: +55-19-3788-3124.

Received 16 August 2002 ,Accepted 21 December 2002.

References 

  1. Li Y, Lafuente A, Trush MA. Characterization of quinone reductase, glutathione and glutathione S-transferase in human myeloid cell lines: induction by 1,2-dithiole-3-thione and effects on hydroquinone-induced cytotoxicity. Life Sci. 1994;54:901–916
  2. Hiruma-Lima CA, Spadari-Bratfisch RC, Kasisse DM, Souza-Brito ARM. Antiulcerogenic mechanisms of dehydrocrotonin, a diterpene lactone obtained from Croton cajucara. Planta Med. 1999;65:325–330
  3. Maciel MAM, Pinto AC, Arruda AC, Pamplona SGSR, Vanderlinde FA, Lapa AJ, et al.  Ethnopharmacology, phytochemistry and pharmacology: a successful combination in the study of Croton cajucara. J. Ethnopharmacol. 2000;70:41–55
  4. Harrison S, Page CP, Spina D. Airway nerves and protein phosphatases. Gen. Pharmacol. 1999;32:287–298
  5. Bialojan C, Takai A. Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatase. Biochemistry. 1988;250:283–290
  6. Riordan FA, Foroni L, Hoffbrand AV, Mehta AB, Wickremasinghe RG. Okadaic acid-induced apoptosis of HL60 leukemia cells is preceded by destabilization of bcl-2 mRNA and downregulation of bcl-2 protein. FEBS Lett. 1998;435:195–198
  7. Gresser MJ, Tracey AS. Vanadate as phosphate analogs in biochemistry. In: Chasteen ND, editor. Vanadium in biological systems. Dordrecht: Kluwer Academic Publishers; 1990. p. 63–79.
  8. Stern A, Yin X, Tsang SS, Davison A, Moon T. Vanadium as a modulator of cellular regulatory cascade and oncogene expression. Biochem. Cell Biol. 1993;71:103–112
  9. Cortizo AM, Bruzzone L, Molinuevo S, Etcheverry SB. A possible role of oxidative stress in the vanadium-induced cytotoxicity in the MC3T3E1 osteoblast and UMR106 osteosarcoma cell lines. Toxicology. 2000;147:89–99
  10. Cadenas E, Davies KJA. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biol. Med. 2000;29:222–230
  11. Huyer G, Liu S, Kelly J, Moffat J, Payette P, Kennedy B, et al.  Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. J. Biol. Chem. 1997;10:843–851
  12. Uzunoglu S, Uslu R, Tobu M, Saydam G, Terzioglu E, Buyukkececi F, et al.  Augmentation of methylprednisolone-induced differentiation of myeloid leukemia cells by serine/threonine protein phosphatase inhibitors. Leuk. Res. 1999;23:507–512
  13. Kohroki J, Muto N, Tanaka T, Itoh N, Inada A, Tanaka K. Induction of differentiation and apoptosis by dithizone in human myeloid leukaemia cell lines. Leuk. Res. 1998;22:405–412
  14. Zhu WH, Majluf-Cruz A, Omburo GA. Cyclic AMP-specific phosphodiesterase inhibitor rolipram and RO-20-1724 promoted apoptosis in HL-60 promyelocytic leukemic cells via cyclic AMP-independent mechanism. Life sci. 1998;63(4):265–274
  15. Wyllie AH, Kerr JF, Currie AR. Cell death: the significance of apoptosis. Int. Rev. Cytol. 1980;68:251–306
  16. Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton GL, Henson PM. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers apecific recognition and removal by macrophages. J. Immunol. 1992;148:2207–2216
  17. Torsoni MA, Viana RI, Ogo SH. Sulphydryl groups and their relation to the antioxidant enzymes of chelonian red blood cells. Biochem. Mol. Biol. Int. 1998;46:147–156
  18. Breitman TR. Growth and differentiation of human myeloid leukemia cell line HL60. Methods Enzymol. 1990;190:118–130
  19. Cohen G, Sun XM, Snowden RT, Dinsdale D, Skilleter DN. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem. J. 1992;286:331–334
  20. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometry detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V. J. Immunol. Methods. 1995;184:39–51
  21. Martin SJ, Reutelingsperger CPM, McGahon AJ, Rader JA, van Schie RGA, LaFace DM, et al.  Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of bcl-2 and Abl. J. Exp. Med. 1995;182:1545–1556
  22. Homburg CH, Haas M, Von dem Borne AJ, Reutelingsperger CP, Roos D. Human neutrophilis lose their surface Fc gamma RIII and acquire annexin V binding sites during apoptosis in vitro. Blood. 1995;85:532–540
  23. Schindl A, Klosner G, Hönigsmann H, Jori G, Calzavara-Pinton PC, Trautinger F. Flow cytometric quantification of UV-indiced cell death in human squamous cell carcinoma-derived cell line: dose and kinects studies. J. Photochem. Photobiol. B: Biol. 1998;44:97–106
  24. Xu K, Thornalley PJ. Involvement of glutathione metabolism in the cytotoxicity of the phenethyl isothiocyanate and its cysteine conjugate to human leukaemia cells in vitro. Biochem. Pharmacol. 2001;61:165–177
  25. Shimizu T, Oka Y, Awai N, Takeda K. Hypophosphorylatio of pRB and repression of cyclin D3 and cdc25A during the granulocytic differentiation of human myeloblastic leukemia ML-1 cells. Leuk. Res. 1999;23:901–907
  26. Steller H. Mechanisms and genes of cellular suicide. Science. 1995;267:1445
  27. Dirsch VM, Stuppner H, Vollmar AM. Helenalin triggers a CD95 death receptor-independent apoptosis that is not affected by overexpression of Bcl-X-L or Bcl-2. Cancer Res. 2001;61(15):5817–5823
  28. Vandre DD, Wills VL. Inhibition of mitosis by okadaic acid: possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase. J. Cell Sci. 1992;101(1):79
  29. Benito A, Lerga A, Silva M, Leon J, Fernandez-Luna JL. Apoptosis of human mieloid leukemia cells induced by an inhibitor of protein phosphatase (okadaic acid) is prevented by Bcl-2 and Bcl-X (L). Leukemia. 1997;11:940–944

PII: S0145-2126(03)00013-4

doi: 10.1016/S0145-2126(03)00013-4

Leukemia Research
Volume 27, Issue 9 , Pages 823-829 , September 2003