Leukemia Research
Volume 27, Issue 7 , Pages 575-582 , July 2003

Measurement of SIL–TAL1 fusion gene transcripts associated with human T-cell lymphocytic leukemia by real-time reverse transcriptase-PCR

  • John D. Curry

      Affiliations

    • Division of Immunology, Department of Molecular and Cellular Biology, University of California at Berkley, 439 Life Sciences Addition, Berkeley, CA 94720-3200, USA
    • ,
  • Martyn T. Smith

      Affiliations

    • Division of Environmental Health Sciences, School of Public Health, University of California, 216 Earl Warren Hall, Berkeley, CA 94720-7360, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-510-642-8770; fax: +1-510-642-0427.

Received 11 February 2002 ,Accepted 4 October 2002.

References 

  1. Aplan PD, Nakahara K, Orkin SH, Kirsch IR. The SCL gene product: a positive regulator of erythroid differentiation. EMBO J. 1992;11:4073–4081
  2. Visvader J, Begley CG, Adams JM. Differential expression of the LYL, SCL and E2A helix–loop–helix genes within the hemopoietic system. Oncogene. 1991;6:187–194
  3. Cross MA, Heyworth CM, Murrell AM, Bockamp EO, Dexter TM, Green AR. Expression of lineage restricted transcription factors precedes lineage-specific differentiation in a multipotent hemopoietic progenitor cell line. Oncogene. 1994;9:3013–3016
  4. Chen Q, Cheng JT, Tasi LH, Schneider N, Buchanan G, Carroll A, et al.  The tal gene undergoes chromosome translocation in T-cell leukemia and potentially encodes a helix–loop–helix protein. EMBO J. 1990;9:415–424
  5. Aplan PD, Lombardi DP, Ginsberg AM, Cossman J, Bertness VL, Kirsch IR. Disruption of the human SCL locus by “illegitimate” V(D)J recombinase activity. Science. 1990;250:1426–1429
  6. Brown L, Cheng JT, Chen Q, Siciliano MJ, Crist W, Buchanan G, et al.  Site-specific recombination of the tal-1 gene is a common occurrence in human T-cell leukemia. EMBO J. 1990;9:3343–3351
  7. Janssen JW, Ludwig WD, Sterry W, Bartram CR. SIL–TAL1 deletion in T-cell acute lymphoblastic leukemia. Leukemia. 1993;7:1204–1210
  8. Bash RO, Hall S, Timmons CF, Crist WM, Amylon M, Smith RG, et al.  Does activation of the TAL1 gene occur in a majority of patients with T-cell acute lymphoblastic leukemia? A Pediatric Oncology Group study. Blood. 1995;86:666–676
  9. Delabesse E, Bernard M, Landman-Parker J, Davi F, Leboeuf D, Varet B, et al.  Simultaneous SIL–TAL1 RT-PCR detection of all tal(d) deletions and identification of novel tal(d) variants. Br. J. Haematol. 1997;99:901–907
  10. Lipkowitz S, Garry VF, Kirsch IR. Interlocus V–J recombination measures genomic instability in agriculture workers at risk for lymphoid malignancies. Proc. Natl. Acad. Sci. U.S.A. 1992;89:5301–5305
  11. Garry VF, Tarone RE, Kirsch IR, Abdallah JM, Lombardi DP, Long LK, et al.  Biomarker correlations of urinary 2,4-D levels in foresters: genomic instability and endocrine disruption. Environ. Health Perspect. 2001;109:495–500
  12. Liu Y, Hernandez AM, Shibata D, Cortopassi GA. BCL-2 translocation frequency rises with age in humans. Proc. Natl. Acad. Sci. U.S.A. 1994;91:8910–8914
  13. Limpens J, Stad R, Vos C, de Vlaam C, de Jong D, van Ommen GJ, et al.  Lymphoma-associated translocation t(14;18) in blood B cells of normal individuals. Blood. 1995;85:2528–2536
  14. Yasukawa M, Bando S, Dolken G, Sada E, Yakushijin Y, Fujita S, et al.  Low frequency of BCL-2/J(H) translocation in peripheral blood lymphocytes of healthy Japanese individuals. Blood. 2001;98:486–488
  15. Uckun FM, Herman-Hatten K, Crotty ML, Sensel MG, Sather HN, Tuel-Ahlgren L, et al.  Clinical significance of MLL–AF4 fusion transcript expression in the absence of a cytogenetically detectable t(4;11)(q21;q23) chromosomal translocation. Blood. 1998;92:810–821
  16. Trka J, Zuna J, Hrusak O, Michalova K, Muzikova K, Kalinova M, et al.  No evidence for MLL–AF4 expression in normal cord blood samples. Blood. 1999;93:1106–1107 [discussion, p. 1108–1110]
  17. Kim-Rouille MH, MacGregor A, Wiedemann LM, Greaves MF, Navarrete C. MLL–AF4 gene fusions in normal newborns. Blood. 1999;93:1107–1108
  18. Biernaux C, Loos M, Sels A, Huez G, Stryckmans P. Detection of major bcr–abl gene expression at a very low level in blood cells of some healthy individuals. Blood. 1995;86:3118–3122
  19. Chen X, Pan Q, Stow P, Behm FG, Goorha R, Pui CH, et al.  Quantification of minimal residual disease in T-lineage acute lymphoblastic leukemia with the TAL1 deletion using a standardized real-time PCR assay. Leukemia. 2001;15:166–170
  20. van Dongen JJ, Macintyre EA, Gabert JA, Delabesse E, Rossi V, Saglio G, et al.  Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 Concerted Action: investigation of minimal residual disease in acute leukemia. Leukemia. 1999;13:1901–1928
  21. Pallisgaard N, Clausen N, Schroder H, Hokland P. Rapid and sensitive minimal residual disease detection in acute leukemia by quantitative real-time RT-PCR exemplified by t(12;21) TEL–AML1 fusion transcript. Genes Chromosomes Cancer. 1999;26:355–365
  22. Curry JD, McHale C, Smith MT. Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts. Biotechniques. 2002;32(4):768–775
  23. Curry JD, McHale C, Smith MT. Factors influencing real-time RT-PCR results: application of real-time RT-PCR for the detection of leukemia translocations. Mol. Biol. Today. 2002;3:79–84
  24. Fuscoe JC, Zimmerman LJ, Lippert MJ, Nicklas JA, O’Neill JP, Albertini RJ. V(D)J recombinase-like activity mediates hprt gene deletion in human fetal T lymphocytes. Cancer Res. 1991;51:6001–6005
  25. Fuscoe JC, Zimmerman LJ, Harrington-Brock K, Burnette L, Moore MM, Nicklas JA, et al.  V(D)J recombinase-mediated deletion of the hprt gene in T lymphocytes from adult humans. Mutat. Res. 1992;283:13–20
  26. Finette BA, Poseno T, Albertini RJ. V(D)J recombinase-mediated HPRT mutations in peripheral blood lymphocytes of normal children. Cancer Res. 1996;56:1405–1412
  27. Wang QF, Lauring J, Schlissel MS. The c-myb binds to a sequence in the proximal region of the RAG2 promoter and is essential for promoter activity in T-lineage cells. Mol. Cell. Biol. 2000;20:9203–9211

PII: S0145-2126(02)00260-6

doi: 10.1016/S0145-2126(02)00260-6

Leukemia Research
Volume 27, Issue 7 , Pages 575-582 , July 2003

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