Leukemia Research
Volume 27, Issue 5 , Pages 455-464 , May 2003

In vitro culture of human acute lymphoblastic leukemia (ALL) cells in serum-free media; a comparison of native ALL blasts, ALL cell lines and virus-transformed B cell lines

  • Øystein Bruserud

      Affiliations

    • Medical Department, Division of Hematology, Institute of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • Corresponding Author InformationCorresponding author. Tel.: +47-55-97-50-00; fax: +47-55-97-29-50.
    • ,
  • Nils Glenjen

      Affiliations

    • Medical Department, Division of Hematology, Institute of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • ,
  • Anita Ryningen

      Affiliations

    • Medical Department, Division of Hematology, Institute of Medicine, Haukeland University Hospital, N-5021 Bergen, Norway
    • ,
  • Elling Ulvestad

      Affiliations

    • Department of Microbiology and Immunology, Section for Immunology, The Gade Institute, The University of Bergen, Bergen, Norway

Received 31 May 2002 ,Accepted 28 September 2002.

References 

  1. Hoelzer D, Gokbuget N. Recent approaches in acute lymphoblastic leukemia in adults. Crit. Rev. Oncol. Hematol. 2000;36:49–58
  2. Berman E. Recent advances in the treatment of acute leukemia: 1999. Curr. Opin. Hematol. 2000;7:205–211
  3. Cortes JE, Kantarjian H, Freireich EJ. Acute lymphoblastic leukemia: a comprehensive review with emphasis on biology and therapy. Cancer Treat. Res. 1996;84:291–323
  4. Shao L-E, Dialynas DP, Yu AL, Mackintosh E, Yu J. Human cord blood conditioned medium enhances leukemia colony formation in vitro. Leuk. Res. 2000;24:1041–1048
  5. Pontvert-Delucq S, Hibner U, Vilmer E, Baillou C, Rohrlich P, Heymann D, et al.  Heterogeneity of B lineage acute lymphoblastic leukemias (B-ALL) with regard to their in vitro spontaneous proliferation, growth factor response and BCL-2 expression. Leuk. Lymphoma. 1996;21:267–280
  6. Srivannaboon K, Shanafelt AB, Todisco E, Forte CP, Behm FG, Raimondi SC, et al.  Interleukin 4 variant (BAY 36-1677) selectively induces apoptosis in acute lymphoblastic leukemia cells. Blood. 2001;97:752–758
  7. Köhler T, Plettig R, Wetzstein W, Schmitz M, Ritter M, Mohr B, et al.  Cytokine-driven differentiation of blasts from patients with acute myelogenous and lymphoblastic leukemia into dendritic cells. Stem Cells. 2000;18:139–147
  8. Duyn AEJ, Kaspers GJL, Pieters R, van Zantwijk CH, Broekema GJ, Hählen K, et al.  Effects of interleukin 3, interleukin 7, and B-cell growth factor on proliferation and drug resistance in vitro in childhood acute lymphoblastic leukemia. Ann. Haematol. 1999;78:163–171
  9. Manabe A, Coustan-Smith E, Behm FG, Raimondi SC, Campara D. Bone marrow derived stromal cells prevent apoptotic cell death in B-lineage acute lymphoblastic leukemia. Blood. 1992;79:2370–2377
  10. Bruserud Ø, Frostad S, Foss B. In vitro culture of acute myelogenous leukemia blasts: a comparison of four different culture media. J. Hematother. 1999;8:63–73
  11. Bradstock KF, Makrynikola V, Bianchi A, Shen W, Hewson J, Gottlieb DJ. Effects of the chemokine stromal cell-derived factor-1 on the migration and localization of precursor-B acute lymphoblastic leukemia ells within the bone marrow. Leukemia. 2000;14:882–888
  12. Petrucci MT, de Felice L, Riccardi MR, Ariola C, Mascolo MG, Fenu S, et al.  Stem cell factor and PIXY-321 is acute lymphoblastic leukemia: in vitro study on proliferation effects and apoptosis. Cytokines Mol. Ther. 1996;2:225–230
  13. Eder M, Hemmati P, Kalina U, Ottman OG, Höltzer D, Lyman SD, et al.  Effects of Flt3-L and interleukin 2 on in vitro growth of acute lymphoblastic leukemia cells. Exp. Hematol. 1996;24:371–377
  14. Campana D, Coustan-Smith E, Kumagai MA, Manabe A. Growth requirements of normal and leukemic human B cell progenitors. Leuk. Lymphoma. 1994;13:359–371
  15. Eder M, Ottmann OG, Hansen-Hagge TE, Bartram CR, Falk S, Gillis S, et al.  In vitro culture of common acute lymphoblastic leukemia blasts: effects of interleukin 3, interleukin 7 and accessory cells. Blood. 1992;79:3274–3284
  16. Makrynikola V, Kabral A, Bradstock KF. Effects of recombinant human cytokines on precursor-B acute lymphoblastic leukemia cells. Exp. Hematol. 1991;19:674–679
  17. Buske C, Hiddemann W, Wormann B. Proliferation of human leukemic pre-B cells induced by human bone marrow stromal cells and murine fibroblasts. Cell Biol. Int. 1994;18:1049–1058
  18. Fortney JE, Zhao W, Wenger SL, Gibson LF. Bone marrow stromal cells regulate caspase 3 activity in leukemic cells during chemotherapy. Leuk. Res. 2001;25:901–907
  19. Bradstock K, Bianchi A, Makrynikola V, Filshie R, Gottlieb D. Long-term survival and proliferation of precursor-B acute lymphoblastic leukemia cells on human bone marrow stroma. Leukemia. 1996;10:813–820
  20. Ashley DM, Bol SJ, Kannourakis G. Viable bone marrow stromal cells are required for the in vitro survival of B-cell precursors acute lymphoblastic leukemic cells. Leuk. Res. 1995;19:113–120
  21. Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, et al.  Proposals for the immunological classification of acute leukemias. Leukemia. 1995;9:1783–1786
  22. Bruserud Ø, Gjertsen BT, von Volkman HL. In vitro culture of acute myelogenous leukemia (AML) cells in serum-free media: studies of native AML blasts and AML cell lines. J. Hematother. Stem. Cell Res. 2000;9:923–932
  23. Bruserud Ø. Effects of dipyridamole, theophyllamine and verapamil on spontaneous proliferation of myelogenous leukemia cells. Acta Oncol. 1992;31:53–58
  24. Bruserud Ø, Gjertsen BT, Brustugun OT, Bassoe CF, Akselsen PE, Bühring HJ, et al.  Effect of interleukin 10 on blast cells derived from patients with acute myelogenous leukemia. Leukemia. 1995;9:1910–1920
  25. Bruserud Ø. Effects of interleukin 13 on cytokine secretion by human acute myelogenous leukemia blasts. Leukemia. 1996;10:1497–1503
  26. Bruserud Ø, Gjertsen BT, Foss B, Huang TS. New strategies in the treatment of acute myelogenous leukemia (AML): in vitro culture of AML cells—the present use in experimental studies and the possible importance for future therapeutic strategies. Stem. Cells. 2001;19:1–11
  27. Bruserud Ø, Ulvestad E. Acute myelogenous leukemia blasts as accessory cells during in vitro T lymphocyte activation. Cell Immunol. 2000;206:36–50
  28. Abrahamsen JF, Bakken AM, Bruserud Ø, Gjertsen BT. Flow cytometric measurement of apoptosis and necrosis in cryopreserved PBPC concentrates from patients with malignant diseases. Bone Marrow Transplant. 2002;29:165–171
  29. Sissolak G, Hoffbrand AV, Mehta AB, Ganeshaguru K. Effects of interferon (IFN) alpha on the expression of interleukin 1 beta (IL1), interleukin 6, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor alpha (TNF) in acute myeloid leukemia blasts. Leukemia. 1992;6:1155–1160
  30. Fiedler W, Suciu E, Wittlief C, Ostertag W, Hossfeld DK. Mechanisms of growth factor expression in acute myeloid leukemia (AML). Leukemia. 1990;4:459–461
  31. Espinoza-Hernandez L, Cruz-Rico J, Benitez-Aranda H, Martinez-Jaramillo G, Rodriguez-Zepeda MC, Velez-Ruelas MA, et al.  In vitro characterization of the hematopoietic system in pediatric patients with acute lymphoblastic leukemia. Leukemia Res. 2001;25:295–303
  32. Bloch A. Dynamics of interaction between DNA-specific antitumor agents and serum-contained cytokines in the initiation of ML-1 human myeloblastic leukemia cell differentiation. Leukemia. 1993;7:1219–1224
  33. Kebelmann-Betzing C, Korner G, Badiali L, Buchwald D, Moricke A, Korte A, et al.  Characterization of cytokine, growth factor receptor, costimulatory and adhesion molecule expression patterns of bone marrow blasts in relapsed childhood B cell precursor ALL. Cytokine. 2001;13:39–50

PII: S0145-2126(02)00227-8

doi: 10.1016/S0145-2126(02)00227-8

Leukemia Research
Volume 27, Issue 5 , Pages 455-464 , May 2003

Article Tools

* Image Usage & Resolution