Alteration in the expression of early stage processing enzymes of N-glycan during myeloid and monocytoid differentiation of HL-60 cells

Abstract 

The expressions of the enzymes participating in the early stage of N-glycan processing, Golgi α-Mase-I, α-Mase-II and GnT-I, GnT-II, were studied before and after HL-60 cells were differentiated to myelocytes or monocytes induced by ATRA or PMA, respectively. It was found that α-Mase-I activity and GnT-I mRNA were decreased by both ATRA and PMA, while α-Mase-II and GnT-II were altered insignificantly. The down-regulation of α-Mase-I and GnT-I was cell specific, since ATRA up-regulated α-Mase-I and GnT-I in the H7721 hepatocarcinoma cell line. However, in H7721 cells, PMA also decreased α-Mase-I and GnT-I, and both ATRA and PMA also did not obviously change the expressions of α-Mase-II and GnT-II.

Abbreviations:  ATRA, all-trans retinoic acid, PMA, phorbol-12-myristate-13-acetate, α-Mase, α-mannosidase, GnT, N-acetylglucosaminyltransferase, GAPDH, 3-phosphoglyceraldehyde dehydrogenase, DMJ, 1-deoxymannojirimycin, SW, swainsonine, Man, mannose, GlcNAc, N-acetylglucosamine, Glc, glucose, Con A, concanavalin A, FCS, fetal calf serum, BSA, bovine serum albumin, PMSF, phenylmethylsulfonyl fluoride, PBS, phosphate buffered saline, EDTA, ethylenediamine tetraacetate, SDS, sodium dodecyl sulfate

Keywords:  Human premyelocytic leukemia cell HL-60, Myeloid differentiation, Monocytoid differentiation, α-Mannosidase, N-acetylglucosaminyltransferase