HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells

Abstract 

K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.

Abbreviations:  MPO, myeloperoxidase, MHC, major histocompatibility complex, IFN-γ, interferon-γ, PMA, phorbol 12-myristate 13-acetate, GCM, growth-conditioned medium, RT-PCR, reverse transcription-polymerase chain reaction, FBS, fetal bovine serum, TEM, transmission electron microscopy, FITC, fluoroscein isothiocyanate, PE, phycoerythrin, KLH, keyhole limpet hemocyanin, ER, endoplasmic reticulum

Keywords:  K-562 cells, Myeloperoxidase, MPO mRNA, MPO protein, Differentiation, Growth-conditioned medium