Phosphomonoester concentrations differ between chronic lymphocytic leukemia cells and normal human lymphocytes

Abstract 

Levels of phospholipid-related metabolites of chronic lymphocytic leukemia lymphocytes (CLL) and normal human lymphocytes were quantified using phosphorus magnetic resonance spectroscopy. The CLL cells versus normal lymphocytes showed significant increases of phosphoethanolamine(Etn-P) (8.11±2.10 mean±S.E., μmol/g wet weight, n=12 versus 3.63±1.10, n=3, P≤0.002), phosphocholine (2.10±0.37, n=12 versus 0.36±0.09, n=3, P≤0.01), glycerophosphoethanolamine (0.26±0.03, n=10 versus 0.11±0.05, n=3, P≤0.004), and glycerophosphocholine (0.33±0.03, n=10 versus 0.17±0.05, n=3, P≤0.003). Further, the phospholipid precursor ethanolamine (Eth) was studied in blood and was found significantly lowered in CLL patients (4.6±1.6 μM, n=25) compared to normal volunteers (7.7±2.5, n=12, P≤0.001). Increased intermediates with depletion of precursors suggest the presence of sustained phospholipid metabolism activation in CLL.

Abbreviations:  CDTA, trans-1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic acid, Cho-P, phosphocholine, CLL, chronic lymphocytic leukemia, Etn, ethanolamine, Etn-P, phosphoethanolamine, EPPS, (N-[2-Hydroxyethyl]piperazine-N’-[3-propanesulfonic acid], GB, Gaussian broadening, Gro-P-Cho, glycerophosphocholine, Gro-P-Etn, glycerophosphoethanolamine, LB, Lorentzian broadening, MRS, magnetic resonance spectroscopy, NDP, nucleoside diphosphates, NHL, non-Hodgkin’s lymphoma, NTP, nucleoside triphosphates, PCA, perchloric acid, PDE, phosphodiesters, PME, phosphomonoesters, PPA, phenylphosphoric acid, RPMI, tissue culture medium originally developed by the Roswell Park Memorial Institute.

Keywords:  Phosphomonoesters, B-cell malignancies, MR spectroscopy, Chronic lymphocytic leukemia, Non-hodgkin’s lymphoma