The Bcl6 locus is not mutated in mouse B-cell lineage lymphomas

Hori, Mitsuo; Qi, Chen-Feng; Torrey, Ted A.; Huppi, Konrad; Morse III, Herbert C.

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Volume 26, Issue 8 , Pages 739-743, August 2002

The Bcl6 locus is not mutated in mouse B-cell lineage lymphomas

Mitsuo Hori
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 7 Centre Drive, Room 304, MSC 0760, Bethesda, MD 20892, USA
- Corresponding author. Tel.: +1-301-496-9529; fax: +1-301-402-0077
Chen-Feng Qi
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 7 Centre Drive, Room 304, MSC 0760, Bethesda, MD 20892, USA
Ted A. Torrey
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 7 Centre Drive, Room 304, MSC 0760, Bethesda, MD 20892, USA
Konrad Huppi
- Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Herbert C. Morse III
- Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 7 Centre Drive, Room 304, MSC 0760, Bethesda, MD 20892, USA

Received 24 April 2001; accepted 29 November 2001.

Abstract

In normal human germinal centre (GC) B-cells and post-GC B-cell lymphomas, a region in the first intron of the BCL6 gene, termed the major mutations cluster (MMC) exhibits somatic point mutations and deletions with patterns very similar to those seen in the variable regions of immunoglobulin (Ig) genes. In studies of mouse post-GC diffuse large cell lymphoma, Burkitt lymphomas, and plasmacytomas, direct sequencing or cold SSCP analyses revealed no mutations within a 686-bp region in Bcl6 intron 1 with 72% identity to the human MMC. The mouse Bcl6 locus must be inaccessible to the mutational machinery responsible for somatic mutations of Ig and BCL6 in humans.

Abbreviations: CB, centroblastic, DLBCL, diffuse large B-cell lymphoma, FL, follicular lymphoma, GC, germinal centre, IB, immunoblastic, IgV region, immunoglobulin gene variable region, MMC, major mutations cluster, MuLV, murine leukaemia virus, NHL, non-Hodgkin’s lymphoma, PCR, polymerase chain reaction, SSCP, single-strand conformational polymorphism