Leukemia Research
Volume 25, Issue 12 , Pages 1047-1050, December 2001

Antibody against CD20 in patients with B cell malignancy

  • Penelope J. Adamson

      Affiliations

    • Child Health Research Institute, Women's and Children's Hospital, University of Adelaide, 72 King William Road, Adelaide 5006, Australia
    • Studies carried out as candidate for PhD degree in the Department of Paediatrics, University of Adelaide. Present Address: Department of Microbiology, Flinders Medical Centre, Adelaide, Australia.
    • ,
  • Heddy Zola

      Affiliations

    • Child Health Research Institute, Women's and Children's Hospital, University of Adelaide, 72 King William Road, Adelaide 5006, Australia
    • Corresponding Author InformationCorresponding author. Tel.: +61-8-82047015; fax: +61-8-82390267
    • ,
  • Ian C. Nicholson
    • ,
  • Glenn Pilkington

      Affiliations

    • Intracel Corp., Rockville, MD, USA
    • ,
  • Arthur Hohmann

      Affiliations

    • Child Health Research Institute, Women's and Children's Hospital, University of Adelaide, 72 King William Road, Adelaide 5006, Australia

Received 11 August 2000; accepted 7 April 2001.

Abstract 

Cancer patients may make antibodies against antigens on the surface of their malignant cells due either to the expression of unique antigens or to dysregulated responses to self antigens. Patients with B cell malignancy frequently produce autoantibodies and may therefore be a source of immunoglobulin genes for the production of phage display antibody libraries directed against tumour-associated antigens. Patients with autoimmune disease have circulating antibodies against lymphocyte surface antigens, and may also provide a good starting point for the production of a library of lymphocyte-reactive antibody structures. In this study, plasma and serum samples from patients with B cell malignancy or Sjogren's syndrome and from healthy controls were screened for antibodies against the B cell membrane antigens CD20. While the majority of samples showed very low reactivity, some individuals did show significant and reproducible binding to CD20. To identify a good donor for library construction, it would be advisable to screen donors for antibody against the antigens of interest.

Keywords:  CD20, Autoantibody, Phage display antibody library

Abbreviations: CLL, chronic lymphocytic leukemia

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PII: S0145-2126(01)00074-1

Leukemia Research
Volume 25, Issue 12 , Pages 1047-1050, December 2001

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