Leukemia Research
Volume 25, Issue 9 , Pages 769-774 , 1 September 2001

Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products

  • Beate Gleissner

      Affiliations

    • Department of Hematology, Oncology and Transfusion Medicine, University Hospital Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-30-84453169; fax: +49-30-84454468
    • ,
  • Juergen Maurer

      Affiliations

    • Department of Hematology, Oncology and Transfusion Medicine, University Hospital Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    • ,
  • Annette Sindram

      Affiliations

    • Department of Hematology, Oncology and Transfusion Medicine, University Hospital Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    • ,
  • Richard Reinhard

      Affiliations

    • Max Planck Institute for Molecular Genetics, Berlin, Germany
    • ,
  • Eckhard Thiel

      Affiliations

    • Department of Hematology, Oncology and Transfusion Medicine, University Hospital Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 Berlin, Germany

Received 20 March 2000 ,Accepted 20 January 2001.

References 

  1. Van Dongen JJM, Wolvers Tettero ILM. Analysis of immunoglobulin and T-cell receptor genes. Part I: Basic and technical aspects. Clin. Chim. Acta. 1991;198:1–91
  2. Achille A, Scarpa A, Montresor M, Scardoni M, Zamboni G, Chilosi M, et al. Routine application of polymerase chain reaction in the diagnosis of monoclonality of B-cell lymphoid proliferations. Diagn. Mol. Pathol. 1995;4:14–24
  3. Aubin J, Davi F, Nguyen-Salomon F, Leboeuf D, Debert C, Taher M, et al. Description of a novel FR1 IgH PCR strategy and its comparison with three other strategies for the detection of clonality in B cell malignancies. Leukemia. 1995;9:471–479
  4. Diss TC, Peng H, Wotherspoon AC, Isaacson PG, Pan L. Detection of monoclonality in low-grade B-cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type. J. Pathol. 1993;169:291–295
  5. Segal GH, Jorgensen T, Scott M, Braylan RC. Optimal primer selection for clonality assessment by polymerase chain reaction analysis: II Follicular lymphoma. Hum. Pathol. 1994;25:1276–1282
  6. Segal GH, Jorgensen T, Masih AS, Braylan RC. Optimal primer selection for clonality assessment by polymerase chain reaction analysis: 1. Low grade B-cell lymphoproliferative disorders of nonfollicular center cell type. Hum. Pathol. 1994;25:1269–1274
  7. Trainor KJ, Brisco MJ, Wan JH, Neoh S, Grist S, Morley AA. Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction. Blood. 1991;78:192–196
  8. Potter MN, Steward CG, Maitland NJ, Oakhill A. Detection of clonality in childhood B-lineage acute lymphoblastic leukemia by the polymerase chain reaction. Leukemia. 1992;6:289–294
  9. Linke B, Bolz I, Fayyazi A, von Hofen M, Pott Ch, Bertram J, et al. Automated high resolution PCR fragment analysis for identification of clonally rearranged immunoglobulin heavy chain genes. Leukemia. 1997;11:1055–1062
  10. Landman-Parker J, Aubin J, Delabesse E, Tabone M-D, Adam M, Millien C, et al. Simplified strategies for minimal residual disease detection in B-cell precursor acute lymphobastic leukemia. Br. J. Haematol. 1996;95:281–290
  11. Tierens A, Lozano R, Wickert R, Chan WC, Greiner TC. High-resolution analysis of immunoglobulin heavy-chain gene rearrangements using denaturing gradient gel electrophoresis. Diagn. Mol. Pathol. 1996;5:159–165
  12. Linke B, Pyttlich J, Tiemann M, Suttorp M, Parwaresch R, Hiddemann W, et al. Identification and structural analysis of rearranged immunogloublin heavy chain genes in lymphomas and leukemias. Leukemia. 1995;9:840–847
  13. Davis TH, Yockey CE, Balk SP. Detection of clonal immunoglobulin gene rearrangements by polymerase chain reaction amplification and single-strand conformational polymorphism analysis. Am. J. Pathol. 1993;142:1841–1847
  14. González M, González D, López-Pérez R, Garcia-Sanz R, Chillón MC, Balanzategui A, et al. Haematologica. 1999;84:779–784
  15. Roth J, Daus H, Trümper L, Gause A, Salamon-Looijen M, Pfreundschuh M. Detection of immunoglobulin heavy-chain gene rearrangement at the single-cell level in malignant lymphomas: no rearrangement is found in Hodgkin and Reed-Sternberg cells. Int. J. Cancer. 1994;57:799–804
  16. Zwicky CS, Maddocks AB, Andersen N, Gribben JG. Eradication of polymerase chain reaction detectable immunoglobulin gene rearrangement in non-Hodgkin lymphoma is associated with decreased relapse after autologous bone marrow transplantation. Blood. 1996;9:3314–3322
  17. Provan D, Bartlett-Pandite L, Zwicky C, Neuberg D, Maddocks A, Corradini P, et al. Eradication of polymerase chain reaction-detectable chronic lymphocytic leukemia cells is associated with improved outcome after bone marrow transplantation. Blood. 1996;88:2228–2235
  18. Rockman SP. Determination of clonality in patients who present with diagnostic dilemmas: a laboratory experience and review of the literature. Leukemia. 1997;11:852–862
  19. Assaf C, Hummel M, Dippel E, Goerdt S, Müller H-H, Anagnostopoulos I, et al. High detection rate of T-cell receptor beta chain rearrangements in T-cell lymphoproliferations by family specific polymerase chain reaction in combination with the GeneScan technique and DNA sequencing. Blood. 2000;96:640–646

PII: S0145-2126(01)00025-X

Leukemia Research
Volume 25, Issue 9 , Pages 769-774 , 1 September 2001

Article Tools

* Image Usage & Resolution