Leukemia Research
Volume 24, Issue 12 , Pages 1041-1048, December 2000

Human cord blood conditioned medium enhances leukemia colony formation in vitro

  • Li-en Shao

      Affiliations

    • Department of Molecular and Experimental Medicine, NX 3, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    • ,
  • Deno P Dialynas

      Affiliations

    • Department of Molecular and Experimental Medicine, NX 3, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    • ,
  • Alice L Yu

      Affiliations

    • Department of Pediatrics, University of California at San Diego Medical Center, San Diego, CA 92103, USA
    • ,
  • Ellen Mackintosh

      Affiliations

    • San Diego Blood Bank, San Diego, CA 92103, USA
    • ,
  • John Yu

      Affiliations

    • Department of Molecular and Experimental Medicine, NX 3, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-858-784-7924

Received 29 December 1999; accepted 12 March 2000.

Abstract 

Childhood T-cell acute lymphoblastic leukemia (T-ALL) is one of the most common childhood cancers. Recently, we observed that pre-conditioning sub-lethally irradiated immunodeficient mice with human cord blood mononuclear cells facilitates in these mice high level engraftment of primary T-ALL cells obtained from patients. Here we report that human cord blood cells secrete a factor(s) which markedly enhances in vitro both colony number and burst size of the T-ALL clonogenic progenitors from patients. The enhancing activity does not correspond to IL-2, IL-15, nor to several other cytokines implicated in T cell proliferation/activation. Thus, it is possible cord blood may secrete an as yet unidentified factor(s) acting on leukemia clonogenic progenitors of T-ALL. Collectively, these studies should prove invaluable in addressing the growth properties of primary T-ALL cells from patients.

Keywords:  Primary leukemia, T-ALL, Cord blood, Leukemia colonies

Abbreviations:  FITC, fluorescein isothiocyanate, MNC, mononuclear cell, NOD/scid mouse, nonobese diabetic×severe combined immunodeficient mouse, PE, phycoerythrin, PMA, phorbol 12-myristate 13-acetate, T-ALL, T-cell acute lymphoblastic leukemia

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PII: S0145-2126(00)00076-X

doi:10.1016/S0145-2126(00)00076-X

Leukemia Research
Volume 24, Issue 12 , Pages 1041-1048, December 2000

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