Granulocyte-colony stimulating factor induced intranuclear endonuclease in murine leukemia cell line

Abstract 

We have reported that murine leukemia cell line (C2M-A5) induced apoptosis by G-CSF. To clarify the mechanism, mRNA expression of apoptosis-related genes was studied. It revealed transient over-expression of c-myc, H-ras and p53 and down-expression of bcl-2. These changes were known as triggers of endonuclease induction. After 96 h culture with G-CSF, apoptosis was occurred simultaneously with endonuclease (37 kd) activation. This endonuclease induced the digestion of double-strand DNA and might be associated with caspase3. Although G-CSF accelerates cell growth and prevents apoptosis in general, it is a contradictory effect. We concluded that G-CSF induced endogenous endonuclease activity in C2M-A5.

Keywords:  G-CSF, Apoptosis, Endonuclease, Myeloid leukemia cells, c-myc, H-ras, p53, bcl-2, Caspase3

Abbreviations:  CAD, caspase3-activated DNase, rh-G-CSF, recombinant human granulocyte-colony stimulation factor